ChloroquineV1, Main, Exploration, bibRecord, 001772

Mesoporous silica nanoparticles enhance MTT formazan exocytosis in HeLa cells and astrocytes

Identifieur interne : 001772 ( Main/Exploration ); précédent : 001771; suivant : 001773

Mesoporous silica nanoparticles enhance MTT formazan exocytosis in HeLa cells and astrocytes

Auteurs : Matthieu Fisichella [France] ; Hinda Dabboue ; Sanjib Bhattacharyya ; Marie-Louise Saboungi ; Jean-Paul Salvetat ; Tobias Hevor ; Martine Guerin [France]

Source :

Toxicology in Vitro [ 0887-2333 ] ; 2009-06.

RBID : Hal:hal-02127511

Abstract

We report on the observation that mesoporous silica nanoparticles (MSNs), after being endocytosed, interfere with the MTT test in HeLa cells and astrocytes by accelerating the exocytosis of formazan crystals. The stimulation of MTT formazan exocytosis is probably related to perturbation of intracellular vesicle trafficking by MSN uptake as revealed by experiments in presence of chloroquine and genistein. Similar effect has been previously observed with a number of chemicals, especially with neurotoxic beta amyloid peptides, but not with nanoparticles. We showed also that MTT reduction test gives an overestimation of the cytotoxicity of mesoporous silica nanoparticles compared to other tests such as LDH activity, WST-1 test and flow cytometry. These findings show that MTT assay should not be used for the study of MSN toxicity, and that perturbation of intracellular trafficking has to be taken into account in evaluating biocompatibility of MSNs.

Url:

https://hal.archives-ouvertes.fr/hal-02127511

DOI: 10.1016/j.tiv.2009.02.007

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">              <p>We report on the observation that mesoporous silica nanoparticles (MSNs), after being endocytosed, interfere with the MTT test in HeLa cells and astrocytes by accelerating the exocytosis of formazan crystals. The stimulation of MTT formazan exocytosis is probably related to perturbation of intracellular vesicle trafficking by MSN uptake as revealed by experiments in presence of chloroquine and genistein. Similar effect has been previously observed with a number of chemicals, especially with neurotoxic beta amyloid peptides, but not with nanoparticles. We showed also that MTT reduction test gives an overestimation of the cytotoxicity of mesoporous silica nanoparticles compared to other tests such as LDH activity, WST-1 test and flow cytometry. These findings show that MTT assay should not be used for the study of MSN toxicity, and that perturbation of intracellular trafficking has to be taken into account in evaluating biocompatibility of MSNs.</p>
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Mesoporous silica nanoparticles enhance MTT formazan exocytosis in HeLa cells and astrocytes

Mesoporous silica nanoparticles enhance MTT formazan exocytosis in HeLa cells and astrocytes

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri